SKU: 1682009013

Rat NPHN (Nephrin) QuickTest ELISA Kit

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Ships within 48 hours · Estimated delivery Jul 16 - Jul 21

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Description

Rat NPHN (Nephrin) QuickTest ELISA KitThe Rat NPHN (Nephrin) QuickTest ELISA Kit measures NPHN in Rat Serum, Plasma, Cell Culture Supernatant, Cell Lysate or Tissue Lysate. This kit was based on sandwich ELISA method. The experiment lasted 120 minutes. Capture antibody was conjugated to an affinity tag that was recognized by a specific antibody coated on the QuickTest plate. Add the Cap Det Ab working solution into each well, then add the standards and pilot samples into individual wells.

The Rat NPHN (Nephrin) QuickTest ELISA Kit measures NPHN in Rat Serum, Plasma, Cell Culture Supernatant, Cell Lysate or Tissue Lysate. This kit was based on sandwich ELISA method. The experiment lasted 120 minutes. Capture antibody was conjugated to an affinity tag that was recognized by a specific antibody coated on the QuickTest plate. Add the Cap/Det Ab working solution into each well, then add the standards and pilot samples into individual wells. If the sample contains NPHN, a capture antibody-NPHN-biotin-detection antibody complex was formed. After incubation, unbound conjugates were removed by wash buffer. HRP-Streptavidin was added. After washing, TMB substrates were added to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. The concentration of NPHN in the sample was calculated by drawing a standard curve. The concentration of the target substance is proportional to the OD450 value.

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SKU: 1682009013

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nina
Waukegan, US
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Bathroom set
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Reviewed in the United States on January 9, 2026
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Christie Smith
San Leandro, US
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High quality for a low price.
I forgot that it was an 8 piece set, so when I received it I was delighted. It is beautiful, well made, and of good quality for a low price. I highly recommend it.
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Omaha, US
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Love this item
These are super cute in my bathroom. Easy to clean. Perfect for a bathroom set.
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Leisa Mueller
Lexington, US
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Néstor William Ángel García
Los Angeles, US
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Reviewed in the United States on September 15, 2025

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