SKU: 19029526540

Human CD59 (CD59 glycoprotein) ELISA Kit

Sale price$234.00 Regular price$260.00
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Ships within 48 hours · Estimated delivery Jul 14 - Jul 19

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Description

Human CD59 (CD59 glycoprotein) ELISA KitThe Human CD59 (CD59 glycoprotein) ELISA Kit measures CD59 in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 3h assay time. The microplate provided in this kit has been precoated with anti CD59 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add HRP detection antibody, then

The Human CD59 (CD59 glycoprotein) ELISA Kit measures CD59 in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody-Sandwich ELISA detection method and takes 3h assay time. The microplate provided in this kit has been precoated with anti CD59 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add HRP-detection antibody, then it binds with CD59 bound to precoated antibody. Wash unbound components and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of CD59 in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.

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SKU: 19029526540

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