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Description
Mouse SEMA4D (Semaphorin-4D) ELISA KitThe Mouse (SEMA4D) Semaphorin 4D ELISA Kit measures Semaphorin 4D in Mouse samples. The plate has been pre coated with Mouse SEMA4D antibody. SEMA4D present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse SEMA4D Antibody is added and binds to SEMA4D in the sample. Then Streptavidin HRP is added and binds to the Biotinylated SEMA4D antibody. After incubation unbound Streptavidin HRP is washed away during
The Mouse (SEMA4D) Semaphorin-4D ELISA Kit measures Semaphorin-4D in Mouse samples. The plate has been pre-coated with Mouse SEMA4D antibody. SEMA4D present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse SEMA4D Antibody is added and binds to SEMA4D in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated SEMA4D antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Mouse SEMA4D. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
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