SKU: 54734552739

Canine IL-12p40 ELISA Kit

Sale price$343.80 Regular price$382.00
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Ships within 48 hours · Estimated delivery Jul 17 - Jul 22

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Description

Canine IL-12p40 ELISA KitThe Canine IL 12p40 ELISA kit is a sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre coated with an antibody specific to Dog IL 12p40. Standards or samples are added to the appropriate microtiter plate wells then with a biotin conjugated antibody specific to Dog IL 12p40. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only

The Canine IL-12p40 ELISA kit is a sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dog IL-12p40. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dog IL-12p40. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dog IL-12p40, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dog IL-12p40 in the samples is then determined by comparing the OD of the samples to the standard curve.

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SKU: 54734552739

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