SKU: 68676279267

SPH (Sphingomyelin) ELISA Kit

Sale price$202.50 Regular price$225.00
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Ships within 48 hours · Estimated delivery Jul 19 - Jul 24

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Description

SPH (Sphingomyelin) ELISA KitThe SPH (Sphingomyelin) ELISA immunoassay kit uses the Competitive ELISA principle. The micro ELISA plate provided in this kit has been pre coated with SPH. Standards or samples are added to the appropriate micro ELISA plate wells then with a biotin conjugated antibody specific to SPH. Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme substrate

The SPH (Sphingomyelin) ELISA immunoassay kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with SPH. Standards or samples are added to the appropriate micro ELISA plate wells then with a biotin-conjugated antibody specific to SPH. Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of SPH in the samples is then determined by comparing the OD of the samples to the standard curve.

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SKU: 68676279267

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4.1 ★★★★★
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Ron
Battle Creek, US
★★★★★ 5
Good squeak
Size: Small, Color: Assorted Color
Healthy squeak and she seems to enjoy the texture and mouth feel
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on August 16, 2025
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Verified Purchase
Jennifer B.
Chelsea, US
★★★★★ 5
Still going strong
Size: Small, Color: Assorted Color
My dogs chew apart EVERYTHING, but all six of these are still in great shape. They prefer and fight over the purple one though for unknown reasons. 🤣
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Reviewed in the United States on October 10, 2025
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Juliane Hayes
Phoenix, US
★★★★★ 4
Loud
Size: Small, Color: Assorted Color
I have mixed feelings about this product. I bought this because my puppy kept breaking the squeaker in his squeaker toys. I wanted something where is the squeaker was more durable. This was definitely more durable, it was also 10 times louder than the squeaker in his squeaker toys. He would squeak it all day and night and I could barely hear anything over it. If you asked my puppy to give the review, I’m sure he’d give it five stars and a glowing review, but for me, it was too loud of a squeaker.
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Reviewed in the United States on April 23, 2025
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Cassy
Dallas, US
★★★★★ 5
Small dog dream toy
Size: Small, Color: Assorted Color
Small dog dream toy
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Reviewed in the United States on March 7, 2026
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Alexa C.
Port Orchard, US
★★★★★ 5
Perfect for Small to Medium Dogs
Size: Small, Color: Assorted Color
Little Louise loves these little toys! Couldn’t be more happy with this purchase. Highly recommend for small to medium dogs!
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Reviewed in the United States on December 25, 2025

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