MS4A4A (membrane-spanning 4-domains, subfamily A, member 4) ELISA Kit (Small Sample)

MS4A4A (membrane-spanning 4-domains, subfamily A, member 4) ELISA Kit (Small Sample)The MS4A4A (membrane spanning 4 domains, subfamily A, member 4) ELISA Kit is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre coated with an antibody specific to Human MS4A4A. Standards or samples are added to the appropriate microtiter plate wells then with a biotin conjugated antibody specific to Human MS4A4A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated.

The MS4A4A (membrane-spanning 4-domains, subfamily A, member 4) ELISA Kit is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MS4A4A. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MS4A4A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human MS4A4A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MS4A4A in the samples is then determined by comparing the OD of the samples to the standard curve.